This is the first attempt to characterize proteins in the supernatant in Ana and Oscar. Oscar was harvested 10/08/2013 representing Day 14 (By Jesse Cole). Ana was harvested on 12/26/2013 representing Day 21. Supernatant was collected by passing though 0.2 um filter. Proteomics analysis was carried out using online trypsin digestion cart to increase sensitivity and reduce sample loss.

Peptide spectrum matches were identified using MSGF+. The database was Hot_Lake_v3_UCC_Oscr_20150513_2015-05-27.fasta ...

See associated Assay

Oscar time course was carried out between 8/19/2013 (Day 7) to 9/9/2013 (Day 28). This is a nitrate only study. Oscar time course samples were received from Jesse Cole. Proteomics sample preparation were done by Marina Gritsenko.

The Excel file contains multiple spreadsheets. It contains a summary of spectral count from all time points and proteins identified in each time point. Each time point has three biological replicates (A B, C). Each biological sample is sum of two technical LC-MS runs ...