This is the first attempt to characterize proteins in the supernatant in Ana and Oscar. Oscar was harvested 10/08/2013 representing Day 14 (By Jesse Cole). Ana was harvested on 12/26/2013 representing Day 21. Supernatant was collected by passing though 0.2 um filter. Proteomics analysis was carried out using online trypsin digestion cart to increase sensitivity and reduce sample loss.

Peptide spectrum matches were identified using MSGF+. The database was Hot_Lake_v3_UCC_Oscr_20150513_2015-05-27.fasta ...