Lentiviral Transduction for EGFR perturbation Wednesday, October 28, 2021 Wiley/Hess Lab, Pacific Northwest National Laboratory Document Last reviewed:

Protocol revised from: 20201106_Protocol for Viral Transduction_SP Final Project WP: NH8697 Description Lentiviral constructs have been prepared by VectorBuilder. Will be transducing 2 cell lines (MCF10A_dCas9-KRAB and MCF10A_dCas9-VPR) with sgRNA libraries to obtain variable EGFR expression. Cell Lines

1. MCF10A dCAS9-KRAB a. Antibiotic selection: ...