Steven Wiley

This investigation is designed to understand the processes and mechanisms involved in the succession of microbial communities isolated fro Hot Lake. Succession is both a natural seasonal process as well as a response to disturbance. We want to understand the general principles that are involved in this process, specifically which species drive the succession process and what roles the individual species play both early and late in succession. The influence of nutrition and environmental factors ...

Treat MCF10A cells (in duplicate) with dilution series of LJH685 (Stock in DMSO): 100µM, 33µM, 10µM, 3µM, 1µM, 0.3µM, 0.1µM, 0µM (control) +/- 3ng/ml EGF for 30 min. Total of 32 samples. Extract and quantify activated Ras activity levels by ELISA.

Expected results: We expect to see an increase in Ras activity as we increase the concentrations of LJH685. We should see this increase in both the presence and absence of EGF but see a much stronger effect with EGF. The 30 min sample time should be ...

Collection of scanning electron microscope images of Ana and Oscar UCCs taken in May of 2012. Shows the cyanobacteria structure and multiple associated heterotrophs.

Lentiviral Transduction for EGFR perturbation Wednesday, October 28, 2021 Wiley/Hess Lab, Pacific Northwest National Laboratory Document Last reviewed:

Protocol revised from: 20201106_Protocol for Viral Transduction_SP Final Project WP: NH8697 Description Lentiviral constructs have been prepared by VectorBuilder. Will be transducing 2 cell lines (MCF10A_dCas9-KRAB and MCF10A_dCas9-VPR) with sgRNA libraries to obtain variable EGFR expression. Cell Lines

1. MCF10A dCAS9-KRAB a. Antibiotic selection: ...

A primary goal of cancer systems biology is to build models of cell regulatory systems that can predict the impact of genetic changes that cause cancer and that can be used to design therapeutic strategies. Unfortunately, the great majority of current signaling models are mostly descriptive and lack the ability to predict responses across different cell types or the impact of gene copy number alterations. To address this critical capability gap, we have developed a measurement-modeling-perturbation ...

Poster presented at the 2019 annual CSBC meeting: A major aim of cancer systems biology is to build models that can predict the impact of these genetic disruptions to guide therapeutic interventions. A prominent driver of cancer cell growth is signaling pathway deregulation from mutations in key regulatory nodes and loss/gain in gene copy number (CNV). Recent work by our group discovered that the abundances of most signaling pathway proteins are highly conserved with signaling being controlled ...

The META Center for Systems Biology will host its second annual symposium in Eugene, Oregon on July 31-August 2, 2015. The Symposium on Host-Microbe Systems Biology will focus on the theme of “Synthesis and Selection of Host-Microbe Systems”. We will explore the properties of host-microbe systems from efforts to build these systems de novo, using approaches of synthetic biology, and to subject these systems to selective pressures, using approaches of experimental evolution. More details about the ...