SEEK ID: https://emsl-seek.pnnl.gov/people/34
Location: United States
ORCID: Not specified
Joined: 28th Jan 2015
Expertise: Not specified
Tools: Not specified
Adaptive Responses (Pacific Northwest National Laboratory) ; Energy and Material Processing (Pacific Northwest National Laboratory) ; Microbial Community Dynamics (Pacific Northwest National Laboratory) ; Reverse Sensitivity Analysis for Identifying Predictive Proteomics Signatures of Cancer (Pacific Northwest National Laboratory)
Related items
A major aim of cancer systems biology is to build models that can predict the impact of these genetic disruptions to guide therapeutic interventions. A prominent driver of cancer cell growth is signaling pathway deregulation from mutations in key regulatory nodes and loss/gain in gene copy number (CNV). Recent work by our group discovered that the abundances of most signaling pathway proteins are highly conserved with signaling being controlled by only a few, low abundance key nodes. The activity ...
Public web page: https://csbconsortium.org/research-projects/reverse-sensitivity-analysis-for-identifying-predictive-proteomics-signatures-of-cancer/
Start date: 1st Apr 2019
End date: 30th Apr 2024
Organisms: Homo sapiens
Research in this project is designed to identify the key driving forces and environmental gradients that control material and energy fluxes through microbial communities and delineate their effects on community composition, structure, and function. We will also develop a mechanistic understanding of energy and carbon partitioning among community members under steady-state and during perturbations. Researchers will investigate the interplay between community spatial organization and function with ...
Public web page: http://www.pnnl.gov/biology/programs/fsfa/research.stm
This project is focused on the functional characteristics and interactions of microbes within communities that determine the dynamics of community composition, function, and spatial organization. It uses an iterative experimental-modeling approach to elucidate principles that drive community dynamics in response to altered environmental conditions and examine the relative contributions of interspecies interactions and dispersive processes to changes in community spatial and functional heterogeneity. ...
Public web page: http://www.pnnl.gov/biology/programs/fsfa/research.stm
This project is designed to identify the general principles governing the adaptive response of individual microbes to environmental perturbations within a community context. It seeks to establish the contribution of rapid, post-transcriptional regulatory processes in the overall adaptive response of communities to environmental stress and determine how responses of individual members contribute to community metabolic homeostasis.
Public web page: http://www.pnnl.gov/biology/programs/fsfa/research.stm
Lentiviral Transduction for EGFR perturbation Wednesday, October 28, 2021 Wiley/Hess Lab, Pacific Northwest National Laboratory Document Last reviewed:
Protocol revised from: 20201106_Protocol for Viral Transduction_SP Final Project WP: NH8697 Description Lentiviral constructs have been prepared by VectorBuilder. Will be transducing 2 cell lines (MCF10A_dCas9-KRAB and MCF10A_dCas9-VPR) with sgRNA libraries to obtain variable EGFR expression. Cell Lines
- MCF10A dCAS9-KRAB a. Antibiotic selection: ...
Creators: Steven Wiley, Becky Hess
Submitter: Steven Wiley
Investigations: 1 hidden item
Studies: 1 hidden item
Assays: 1 hidden item
Poster presented at the 2019 annual CSBC meeting: A major aim of cancer systems biology is to build models that can predict the impact of these genetic disruptions to guide therapeutic interventions. A prominent driver of cancer cell growth is signaling pathway deregulation from mutations in key regulatory nodes and loss/gain in gene copy number (CNV). Recent work by our group discovered that the abundances of most signaling pathway proteins are highly conserved with signaling being controlled ...
Creators: Steven Wiley, Herbert Sauro, Becky Hess, WEIJUN QIAN
Submitter: Steven Wiley