2 items tagged with 'genome annotation'.
Projects: Energy and Material Processing, Microbial Community Dynamics, Adaptive Responses
Institutions: Pacific Northwest National Laboratory
Methionine metabolism and uptake genes in Proteobacteria are controlled by a variety of RNA and DNA regulatory systems. We have applied comparative genomics to reconstruct regulons for three known … transcription factors, MetJ, MetR, and SahR, and three known riboswitch motifs, SAH, SAM-SAH, and SAM_alpha, in approximately 200 genomes from 22 taxonomic groups of Proteobacteria. We also identified two novel regulons: a SahR-like transcription factor SamR controlling various methionine biosynthesis genes in the Xanthomonadales group, and a potential RNA regulatory element with terminator-antiterminator mechanism controlling the metX or metZ genes in beta-proteobacteria. For each analyzed regulator we identified the core, taxon-specific and genome-specific regulon members. By analyzing the distribution of these regulators in bacterial genomes and by comparing their regulon contents we elucidated possible evolutionary scenarios for the regulation of the methionine metabolism genes in Proteobacteria.
Authors: S. A. Leyn, I. A. Suvorova, T. D. Kholina, S. S. Sherstneva, P. S. Novichkov, M. S. Gelfand, D. A. Rodionov
Date Published: 20th Nov 2014
Publication Type: Not specified
PubMed ID: 25411846
Citation: PLoS One. 2014 Nov 20;9(11):e113714. doi: 10.1371/journal.pone.0113714. eCollection 2014.
Created: 7th May 2015 at 20:07