Homo sapiens
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Strain details
| Name | Provider name | Provider's strain ID | Genotypes | Phenotypes | Synonym | Comments | Based on |
|---|---|---|---|---|---|---|---|
| MCF 10A | ATCC | CRL-10317 | wild-type | Epithelial | MCF-10A | The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. The calcium content of the medium exerts a strong effect on the morphology of the cells. | Not specified |
| MCF10A dCAS9-VPR | Not specified | Not specified | Vector expression dCas9-VPR | wild-type | MCF10A-CRISPRa | Selection with 500 µg/ml G418 | MCF 10A |
Related items
A major aim of cancer systems biology is to build models that can predict the impact of these genetic disruptions to guide therapeutic interventions. A prominent driver of cancer cell growth is signaling pathway deregulation from mutations in key regulatory nodes and loss/gain in gene copy number (CNV). Recent work by our group discovered that the abundances of most signaling pathway proteins are highly conserved with signaling being controlled by only a few, low abundance key nodes. The activity ...
Public web page: https://csbconsortium.org/research-projects/reverse-sensitivity-analysis-for-identifying-predictive-proteomics-signatures-of-cancer/
Start date: 1st Apr 2019
End date: 30th Apr 2024
Treat MCF10A cells (in duplicate) with dilution series of LJH685 (Stock in DMSO): 100µM, 33µM, 10µM, 3µM, 1µM, 0.3µM, 0.1µM, 0µM (control) +/- 3ng/ml EGF for 30 min. Total of 32 samples. Extract and quantify activated Ras activity levels by ELISA.
Expected results: We expect to see an increase in Ras activity as we increase the concentrations of LJH685. We should see this increase in both the presence and absence of EGF but see a much stronger effect with EGF. The 30 min sample time should be ...
Submitter: Steven Wiley
Assay type: Inhibitor study
Technology type: ELISA
Investigation: 1 hidden item
Study: 1 hidden item
Submitter: Steven Wiley
Provider Name: Not specified
Provider's strain ID: Not specified
Organism: Homo sapiens
Genotypes: Vector expression dCas9-VPR
Phenotypes: wild-type
Comment: Selection with 500 µg/ml G418
Genotypes: wild-type
Phenotypes: Epithelial
Comment: The MCF 10A cell line is a non-tumorigenic epithelial cell line. The cells are positive for epithelial sialomucins, cytokeratins and milk fat globule antigen. They exhibit three dimensional growth in collagen, and form domes in confluent cultures. Thus far, the cells have shown no signs of terminal differentiation or senescence. The line is responsive to insulin, glucocorticoids, cholera enterotoxin, and epidermal growth factor (EGF). By electron microscopy the cells display characteristics of luminal ductal cells but not of myoepithelial cells. They also express breast specific antigens as detected by positive reaction with MFA-Breast and MC-5 monoclonal antibodies. The calcium content of the medium exerts a strong effect on the morphology of the cells.
