Multi-Omic Dynamics Associate Oxygenic Photosynthesis with Nitrogenase-Mediated H2 Production in Cyanothece sp. ATCC 51142

Abstract:

To date, the proposed mechanisms of nitrogenase-driven photosynthetic H2 production by the diazotrophic unicellular cyanobacterium Cyanothece sp. ATCC 51142 have assumed that reductant and ATP requirements are derived solely from glycogen oxidation and cyclic-electron flow around photosystem I. Through genome-scale transcript and protein profiling, this study presents and tests a new hypothesis on the metabolic relationship between oxygenic photosynthesis and nitrogenase-mediated H2 production in Cyanothece 51142. Our results show that net-positive rates of oxygenic photosynthesis and increased expression of photosystem II reaction centers correspond and are synchronized with nitrogenase expression and H2 production. These findings provide a new and more complete view on the metabolic processes contributing to the energy budget of photosynthetic H2 production and highlight the role of concurrent photocatalytic H2O oxidation as a participating process.

SEEK ID: https://emsl-seek.pnnl.gov/publications/25

PubMed ID: 26525576

Projects: Adaptive Responses, Energy and Material Processing, Microbial Community Dynamics

Publication type: Not specified

Journal: Sci Rep

Citation: Sci Rep. 2015 Nov 3;5:16004. doi: 10.1038/srep16004.

Date Published: 4th Nov 2015

Registered Mode: Not specified

Authors: Hans Bernstein, M. A. Charania, R. S. McClure, N. C. Sadler, M. R. Melnicki, E. A. Hill, L. M. Markillie, C. D. Nicora, A. T. Wright, M. F. Romine, A. S. Beliaev

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Created: 8th Apr 2016 at 15:39

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