Lentiviral Transduction for EGFR perturbation Wednesday, October 28, 2021 Wiley/Hess Lab, Pacific Northwest National Laboratory Document Last reviewed:
Protocol revised from: 20201106_Protocol for Viral Transduction_SP Final Project WP: NH8697 Description Lentiviral constructs have been prepared by VectorBuilder. Will be transducing 2 cell lines (MCF10A_dCas9-KRAB and MCF10A_dCas9-VPR) with sgRNA libraries to obtain variable EGFR expression. Cell Lines
- MCF10A dCAS9-KRAB a. Antibiotic selection: ...
Investigations: 1 hidden item
Studies: 1 hidden item
Assays: 1 hidden item
Poster presented at the 2019 annual CSBC meeting: A major aim of cancer systems biology is to build models that can predict the impact of these genetic disruptions to guide therapeutic interventions. A prominent driver of cancer cell growth is signaling pathway deregulation from mutations in key regulatory nodes and loss/gain in gene copy number (CNV). Recent work by our group discovered that the abundances of most signaling pathway proteins are highly conserved with signaling being controlled ...
Submitter: Steven Wiley